Enhanced proliferation of T cell precursors on LmDL1-FL7.A, Lentivector constructs expressing mouse DL1, human IL-7 and human Flt3L. B, qRT-PCR analysis for DL-1, Flt3L, and IL-7. C &D, Flow cytometry analysis of mDL-1 (C) and Flt3L (D) surface expression in the various OP9 cell lines. ELISA analyses for Flt3L (D) and IL-7 (E) production. F, Growth kinetics of developing T cells from adult human BM-derived CD34+ HPCs on LmDL + IL-7 + Flt3L versus LmDL1-FL7 with p value indicated.
Patel et al. BMC Immunology 2012 13:46 doi:10.1186/1471-2172-13-46