Table 1

Primer used for Reverse Transcriptase (RT-PCR)-polymerase chain reaction of cytokines, death receptors, and apoptosis markers in HME patients and controls
Name Primer sequence Product size (bp) GenBank Accession numbers
TLR2 F: 5'-GGA AGA ATC CTC CAA TCA GGC-3' 100 NM_003264
R: 5'-CTT CTG TGA GCC CTG AGG GA-3'
TLR4 F: 5'-AGC CAC GCA TTC ACA GGG–3', 100 NM_138554
R: 5'-CAT GGC TGG GAT CAG AGT CC-3'
TLR-9 F: 5’- GTG ACA GAT CCA AGG TGA AGT-3’ 487 NM_017442
R: 5’- CTT CCT CTA CAA ATG CAT CAC T-3’
Fas F: 5-TGA AGG ACA TGG CTT AGA AGT G-3’ 117 NM_000043
R: 5-GGT GCA AGG GTC ACA GTG TT-3’
FasL F: 5- GCA GCC CTT CAA TTA CCC AT-3’ 100 NM_000639
R: 5-CAG AGG TTG GAC AGG GAA GAA-3’
TNFR p55 F: 5'-TGT GTC TCC TGT AGT AAA TG-3' 589 NM_001065
R: 5'-ACG AAT TCC TTC CAG CGC AA-3'
GAPDH F: 5-GAA GGT GAA GGT CGG AGT C-3’ 225 NM_002046
R: 5-GAA GAT GGT GAT GGG ATT TC-3’

Ismail et al.

Ismail et al. BMC Immunology 2012 13:26   doi:10.1186/1471-2172-13-26

Open Data