Expression of SOCS3 mRNA in monocytes. Monocytes from healthy donors were cultured in 5.5 or 50 mmol/l D-glucose for 2, 24, or 48 hours or 7 days, and the expression levels of SOCS3 mRNA and 18 S ribosomal RNA were evaluated using the Taqman gene assay. Gene expression was normalized to that of the housekeeping gene, and the results are expressed according the ΔΔCt method as the relative fold-change with respect to cells treated with 5.5 mmol/l D-glucose. The data are presented as the mean ± SD (n = 3). * P < 0.05.
Gonzalez et al. BMC Immunology 2012 13:19 doi:10.1186/1471-2172-13-19