Involvement of signal transduction pathways on differentially regulated genes. Jurkat T cells were stimulated as indicated previously and the involvement of different signaling pathways on gene regulation was elucidated using inhibitors of specific pathways, including Lck (1 μM), PKC (10 μM), Calcineurin (1 μM), and MAPKs p38, JNK, and MEK/ERK (all 10 μM). A; Regulation of CCL1 mRNA in Jurkat T cells after stimulation with PMA/CD28-, CD3/28- or PMA/CD3-and co-incubated and cultured for 8 hours in the presence or absence of signal transduction pathway inhibitors. B; Regulation of IL-2 mRNA in Jurkat T cells after stimulation with PMA/CD28-, CD3/28- and PMA/CD3- and co-incubated and cultured for 8 hours in the presence or absence of signal transduction pathway inhibitors. The height of the bars represent the average intensity of 3 biological replicates, the error bars indicate the variation (min and max values). Significance of changes in expression level between the stimulated sample and the sample that was stimulated in the presence of the pathway inhibitor is indicated as follows; *** P value < 0.0001; ** 0.01 <P value > 0.001* 0.05 <P value > 0.01.
Smeets et al. BMC Immunology 2012 13:12 doi:10.1186/1471-2172-13-12