Figure 2.

Expression of prolactin receptor in different subsets of splenic B cells from C57BL/6 mice. The different B cell subsets from 9-week-old C57BL/6 mice were purified by flow cytometry in four independent experiments using eight mice per experiment. Using real-time PCR, PRL receptor (PRL-R) mRNA expression was determined in the following different subsets of splenic B cells: A) Follicular (FO), marginal zone (MZ), and transitional (T). The protein expression levels of the PRL receptor were determined for ten mice of each strain by flow cytometry, and the mean fluorescence intensity (MFI) is shown in the following B cell subsets: B) Follicular; marginal zone; and transitional. C) Histograms of PRL receptor expression in the different subsets of B cells are shown. D) Transitional 1, 2 and 3. The asterisks denote statistical significance with the p value shown.