Table 3

Primary antibodies

Antigen

dilution radio

antigen retrieval

source

Temperature of incubation of primary antibody

Time of incubation of primary antibody

Time of DAB

Time of staining with hematoxylin


CD4

1:20

citrate buffer solution

pressure cooking (pH 6.0) 2 min

Rabbit

37°C

1 hr

6 min

5 min

IL-17

1:100

Tris-EDTA(pH 9.0) 15 min

Rabbit

37°C

1 hr

8 min

5 min

Foxp3

1:100

Tris-EDTA(pH9.0) 15 min

Rat

37°C

1 hr

10 min

2 min

CD8

1:50

Citrate buffer solution

pressure cooking(pH 6.0) 2 min

Rabbit

4°C

Overnight

8 min

3 min

perforin

1:10

EDTA(pH 8.0) pressure cooking 2 min

Mouse

4°C

Overnight

8 min

3 min


Niu et al. BMC Immunology 2011 12:47   doi:10.1186/1471-2172-12-47

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