Infiltration of Hepa1-6 tumors by distinct TAM subsets. (A) Identification of distinct TAM subsets in single-cell suspensions obtained from tumors on Day 21 after tumor inoculation (n = 5). (B) Phenotypic analysis of classically versus alternatively activated TAM subsets using qRT-PCR. Data are representative of three independent experiments. The bars represent the mean ± SD. (C) Expression of IL-10 by sorted TAMs in tumors on Day 10 after tumor inoculation (n = 5). (D) Dynamic analysis of two distinct subsets from tumors on Days 7, 14, 21, and 28 after tumor inoculation. Tumor weights are shown above the FACS plots (n = 5). (E) Number of TAMs (F4/80+MHC IIlow cell population versus F4/80+MHC IIhi cell population) per tumor at the indicated time points. (F) Expression of MHC class II by untreated and TAM- depleted tumors analyzed by immunohistochemistry. Insets: representative cells expressing high and low levels of MHC class II (n = 5) (× 400 magnification). (G) Analysis of MHC class IIhi and MHC class IIlow TAMs in untreated and TAM-depleted tumors on Day 20 after treatment with Cl2MDP-liposomes (n = 5). (H) Proportion of MHC class IIhi and MHC class IIlow TAMs in the untreated and TAM-depleted tumors (n = 5). The bars represent the mean ± SD; **p <0.01, ***p < 0.001 (unpaired Student's t-test).
Wang et al. BMC Immunology 2011 12:43 doi:10.1186/1471-2172-12-43