Email updates

Keep up to date with the latest news and content from BMC Immunology and BioMed Central.

Open Access Highly Accessed Research article

Immunoregulatory effects of AFP domains on monocyte-derived dendritic cell function

Agus Setiyono*, Akterono D Budiyati, Sigit Purwantomo, Madonna R Anggelia, Ismail Fanany, Gunawan A Wibowo, Indra Bachtiar, Andi Utama and Susan Tai

Author Affiliations

Mochtar Riady Institute for Nanotechnology, Tangerang 15810, Indonesia

For all author emails, please log on.

BMC Immunology 2011, 12:4  doi:10.1186/1471-2172-12-4

Published: 17 January 2011

Abstract

Background

Alpha-fetoprotein (AFP) is a tumor-associated glycoprotein that functions in regulation of both ontogenic and oncogenic growth. Recent study showed that AFP can induce apoptosis or impair monocyte-derived dendritic cell (MDDC) function. However, it is still unclear which AFP domain (D-AFP) plays major role in this function.

Results

As expected monocytes cultured in the presence of Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) and Interleukin-4 (IL-4) developed into MDDC. Up-regulation of HLA-DR and CD11c as well as loss of CD14 molecules could be observed. Full length AFP (FL-AFP), domain 2 AFP (D2-AFP) and D3-AFP, but not D1-AFP, significantly inhibited the expression of HLA-DRhigh/CD11chigh and CD80+/CD86high molecules. In contrast, CD83 expression was substantially down-regulated in all samples. Expression of CD40 was significantly suppressed by FL-AFP but not by any D-AFPs. Finally, both FL-AFP and D-AFP impaired the MDDC ability to secrete IL-12 (p70).

Conclusions

D2- and D3- but not D1-AFP extensively suppresses the MDDC function. All the recombinant AFP proteins impaired the ability of MDDC to secrete IL-12.