Transcription of HLA in leukocyte subsets from two humans. We normalized sequence reads for each allele within each leukocyte subset by dividing the number of reads from each class I sequence by the total number of reads identified from that subset. Plots show the mean and points represent independent PCR reactions using distinct MID tags sequenced in a single run. Alleles were distinguished at the two-digit level. a) Normalized transcript levels for the first subject. b) Normalized transcript levels for the second subject. Plots show both data points and the mean. c) Percentage that each subset contributes to the whole PBMC. For CD20 cells, data points from the PCR reaction with MID 1 are shown with red squares while those from MID 2 are shown with red squares and a black outline. A chi-square test was used to compare gene transcription between subsets for each cDNA species.
Greene et al. BMC Immunology 2011 12:39 doi:10.1186/1471-2172-12-39