Table 1

Inhibition of serum IgG from uncomplicated T1D, complicated T1D and control subjects towards native and modified GAD65.

Maximum percent inhibition at 20 μg/ml

Serum

Uncomplicated T1D

Nephropathic T1D

Retinopathic T1D

Controls

N-GAD65

M-GAD65

N-GAD65

M-GAD65

N-GAD65

M-GAD65

N-GAD65

M-GAD65


1

36

54

40

78

39

85

-

-

2

38

40

42

66

37

79

-

7

3

47

61

54

79

44

57

7

11

4

37

55

41

70

41

68

-

-

5

43

49

48

66

32

65

-

-

6

35

54

35

74

37

79

-

10

7

40

60

44

56

43

76

10

7

8

26

68

39

73

51

57

-

-

9

22

37

30

59

36

70

7

9

10

38

56

37

62

50

77

8

5

11

45

63

42

57

40

70

-

7

12

34

50

29

59

48

69

-

-

13

46

55

37

76

50

79

5

9

14

41

52

36

69

36

75

-

-

15

33

39

39

78

33

81

-

-

16

21

48

28

79

30

83

6

7

17

19

41

26

69

28

78

8

6

18

26

37

27

75

37

79

6

7

19

31

45

35

81

35

82

9

8

20

28

47

39

80

40

80

-

-


Mean ± SD

35.2 ± 5.9

50.6 ± 7.2

39.2 ± 5.4

70.3 ± 8.2*

41.1 ± 5.3

74.5 ± 6.5*

7.3 ± 3.6

7.2 ± 3.7


N-GAD65 and M-GAD65 represents Native GAD65 and modified GAD65.

NH represents normal humans as control.

The ELISA plates were coated with N-GAD65 and M-GAD65 (20 μg/ml).

ROS-GAD65 and N-GAD65 were used as inhibitor.

* p < 0.001 vs ROS-GAD65Abs in uncomplicated T1D.

Khan et al. BMC Immunology 2011 12:19   doi:10.1186/1471-2172-12-19

Open Data