Increased proliferation and decreased caspase-3-mediated apoptosis in 129S5 BMMC. (a) Representative flow cytometry plot (day 8) of CFSE-labeled BALB/c and 129S5 BMMCs. Divisional index of 129S5 and BALB/c BMMCs cultured in IL3 (20ng/ml) and SCF (10ng/ml) and CFSE-fluoresence decay was measured on day 4 and day 8 and analyzed by proliferation tool of Flowjo software to determine division index. (b) Percentage of 7AAD- Annexin V+ and (c) active caspase-3+ 129S5 and BALB/c BMMCs (5-week old) cultured in IL-3 (20 ng/ml) and SCF (10 ng/ml). Data represent mean ± SEM and is representative of three separate experiments. (a) Data were analyzed by one-way analysis of variance (ANOVA) and a post-hoc comparison test (Tukey-Kramer). Single and double asterisks indicate a P value < 0.05 and < 0.01, respectively. (b and c) data were analyzed using a Students T-test. Double and triple asterisks indicate a P value < 0.01 and < 0.001, respectively.
Arumugam et al. BMC Immunology 2011 12:14 doi:10.1186/1471-2172-12-14