IgE-mediated BMMC degranulation in 129S5 and BALB/c mice. (a) β-hexosaminidase activity at 15 minutes following IgE-mediated degranulation of 129S5 and BALB/c BMMC. 4 week-cultured 129S5 and BALB/c BMMCs (5 × 106/ml) were sensitized with IgE-TNP (10 μg/ml) and challenged with BSA-TNP for 15 minutes and supernatant was assayed for hexosaminidase activity as described in materials and methods. Data represents mean ± SEM and is representative of three separate experiments. Data were analyzed by one-way analysis of variance (ANOVA) and a post-hoc comparison test (Tukey-Kramer). Double asterisks indicate a P value < 0.01 compared with negative control (IgE and BSA-TNP negative).
Arumugam et al. BMC Immunology 2011 12:14 doi:10.1186/1471-2172-12-14