Table 1

Primers for Quantitative Real-time PCR

Name of gene

Name of primer

Primer sequence

cDNA length (bp)

PGRP

HaPGRPF

HaPGRPR

5'-ccagatgtgctgagatcgtg-3'

5'-tttgccgttaccacctacaa-3'

101

PGRP C

HaPGRPcF

HaPGRPcR

5'-agctgaggtacccacagacg-3'

5'-ccagcccaagctgttatgat-3'

151

GNBP

HaGNRPF

HaGNRPR

5'-caccttgcattacggacctt-3'

5'-tgttacacgtcccagttcca-3'

166

galiomicin

HaGaliF

HaGaliR

5'-tggtgaagagctaccgttcc-3'

5'-acgcagctaccaatcagctt-3'

109

gloverin

HaGloF

HaGloR

5'-gcaagacatcttcaacgacca-3'

5'-tccttgtacacatcaagactgg-3'

150

lysozyme

HaLysF

HaLysR

5'-gaaggactgcaatgttacttg-3'

5'-gcctcgaacttgtggcgtttg-3'

99

Cecropin-1

HaCecF

HaCecR

5'-gtttggtagcagcgtgcag-3'

5'-gcttcaccgaggactgctat-3'

136

cecropin-2

HaCecAF

HaCecAR

5'-tgtcttcgcttgttttgtgg-3'

5'-atcacgaatgtgctgaccaa-3'

103

cecropin 3

HaCecBF:

HaCecBR

5'-gttgttcgtgttcgcgtgt-3'

5'-accgtccctgatgttacgac-3'

112

moricin

HaMorF

HaMorR

5'-gcattactggtgccatctga-3'

5'-ctatgttgatcgcccggagt-3'

103

cobatoxin

HaCobF

HaCobR

5'-tgtgctagttgttataagtgccatt-3'

5'-ctacctgcaccgagttgtca-3'

122

attacin

HaAttF

HaAttR

5'-gagtgggagcttcattaggg-3'

5'-cgaggagcgttaaagtccag-3'

120

gallerimycin

Ha-GallF1

Ha-GallR1

5'-acaagggccacctcttccag-3'

5'-aagtgcagtatccgccagac-3'

92

immune inducible protein

HaIipF

HaIipR

5'-cttatagggtgcgaccaacg-3'

5'-acgtccgagttacagcgaag-3'

161

Ha-β-actin

HaActinF

HaActinR

5' -cctggtattgctgaccgtatgc-3'

5' -ctgttggaaggtggagagggaa-3'

150

Wang et al. BMC Immunology 2010 11:9   doi:10.1186/1471-2172-11-9