Research article
Intensive chemotherapy for acute myeloid leukemia differentially affects circulating TC1, TH1, TH17 and TREG cells
-
* Corresponding author: Elisabeth Ersvaer elisabeth.ersvar@med.uib.no
1 Institute of Medicine, University of Bergen, Bergen, Norway
2 Department of Medicine, Haukeland University Hospital, Bergen, Norway
BMC Immunology 2010, 11:38 doi:10.1186/1471-2172-11-38
Published: 9 July 2010Additional files
Additional file 1:
Release of IL17-A by T cells derived from AML patients with chemotherapy-induced cytopenia. Peripheral blood leukocytes were cultured in the whole blood assay and IL17-A levels determined in the culture supernatants. The leukocytes were cultured in medium alone (CTR), or medium with aCD3 + aCD28 or aCD3 + aCD28 + IL2. A subset of samples were also added the PKC agonist Pep005 in combination with aCD3 + aCD28 + IL2 (denoted Pep005). Results are presented as the cytokine concentration for each sample. Grey circles represent levels below the minimum detectable concentration (< 15 pg/mL), and black circles represent detectable levels.
Format: DOC Size: 760KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional file 2:
IL17-A receptor expression and phosphorylation of intracellular mediators following IL17-A stimulation. Primary leukemia cells derived from 13 untreated AML patients (AML 1-13) were assessed for IL17-A receptor (IL17-R) expression (grey area) by flow cytometry. The lower part of the figures shows the percentages of IL17-R+ cells out of total viable leukemia cells (IL17-R %) and mean fluorescence intensity (MFI) of the IL17-R positive cells (MFI+) versus the IL17-R negative cells (MFI-). The primary human AML cells were also stimulated (Stim), or not stimulated (Unstim), with IL17-A for 5 minutes before cells were assessed for phosphorylation status of Stat3, Stat5, p38, Erk, Creb and Akt. The results are presented as histogram overlay of unstimulated and stimulated samples with color designating fold change (-0.5 - 0.5).
Format: DOC Size: 993KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional file 3:
The effect of IL17-A on spontaneous and cytokine-dependent AML cell proliferation, a summary of the results for 59 consecutive patients. Primary human AML cells were cultured in serum-free medium alone, with IL17-A, or with IL17-A in combination with the indicated exogenous cytokines. AML cell proliferation was assayed as 3H-thymidine incorporation after 7 days of culture in vitro.
Format: DOC Size: 46KB Download file
This file can be viewed with: Microsoft Word Viewer