Induction of regulatory phenotype on T cells depends on IL-10. A and B. Lymph node cultures were stimulated with HPV16 E6 and E7 peptides, 15 μg each, for 6 days. Cells were harvested and stained for CD4 (A), CD8 (B) and Foxp3 and analyzed by flow cytometry. For each sample, 105 lymphocytes were acquired. Lymph nodes were harvested from irrelevant IgG treated mice (IgG), anti-IL10R treated mice (IL10R) or TC-1 tumors bearing mice untreated (TC-1) 18 days after TC-1 cells injection. C. Flow cytometry analysis of peripheral lymph node suspensions stained with the indicated antibodies. Lymph node single cell suspensions were stained with antibodies against the indicated markers and analyzed by flow cytometry. At least 30000 events were acquired per condition. The results are the average of 6 mice. Naïve are cells obtained from mice never exposed to TC-1 tumors; TC-1 corresponds to cells obtained from untreated mice injected with TC-1 cells; IgG and IL-10 correspond to cells obtained from mice with tumors treated with irrelevant IgG and anti-IL-10 and anti-IL-10R, respectively. Asterisks indicate p < 0.05 in t-test.
Bolpetti et al. BMC Immunology 2010 11:27 doi:10.1186/1471-2172-11-27