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Open Access Highly Accessed Research article

The evaluation of metallothionein expression in nasal polyps with respect to immune cell presence and activity

Magdalena Dutsch-Wicherek1*, Romana Tomaszewska2, Agata Lazar2, Paweł Stręk1, Łukasz Wicherek3, Krzysztof Piekutowski3 and Wojciech Jóźwicki4

Author Affiliations

1 Otolaryngology Head and Neck Surgery Department, Jagiellonian University, Sniadeckich 2, Krakow 31-531, Poland

2 Pathomorphology Department, Jagiellonian University, Grzegorzecka, 31-530 Krakow, Poland

3 Gynecology and Oncology Department of the Lukaszczyk Oncological Center in Bydgoszcz and Chair of Gynecology, Oncology and Gynecological Nursing of the Ludwik Rydygier Medical College in Bydgoszcz, Mikolaj Kopernik University, Poland

4 Department of Tumor Pathology and Pathomorphology the L. Rydygier Collegium Medicum UMK F. Lukaszczyk Oncology Center, Bydgoszcz Poland

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BMC Immunology 2010, 11:10  doi:10.1186/1471-2172-11-10

Published: 9 March 2010

Abstract

Background

The expression of metallothionein (MT) is involved in acquiring resistance to immune-mediated apoptosis; it is also a negative regulator of the immune response. Nasal polyps are typified by a resistance to immune-mediated apoptosis as well as by excessive immune cell infiltration. RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) is a membrane protein capable of inducing the apoptosis of CTLs and NK cells. The aim of the present study has been to explore the expression of metallothionein with respect to immune cell presence and immune cell activity. In our study, we identified immune cells using CD4 and CD68 antigen expression and evaluated their activity using CD25 antigen expression. We then analyzed metallothionein, RCAS1, CD25, CD4, and CD68 in a sampling of 50 nasal polyps using the immunohistochemistry method. We were able to divide the nasal polyps into three main groups according to their predominant immune cell infiltration: eosinophilic nasal polyps (21 cases), lymphocytic nasal polyps (17 cases), and neutrophilic nasal polyps (12 cases).

Results

In the present study, statistically significant differences between the MT expression in the epithelium and that in the stroma of the nasal polyps along with the accompanying alterations in activation markers on immune cells were found and the number of macrophages in both the eosinophilic and the lymphocytic nasal polyps was assessed. RCAS1-expressing macrophages were found only in the eosinophilic nasal polyps.

Conclusion

MT expression seems to favor the survival of nasal polyp epithelial cells in the adjacent area of increasingly cytotoxic immune activity. RCAS1-expressing macrophages seem to participate in creating the immune suppressive microenvironment and so help to sustain local inflammation.