Schema for isolating sCTLA-4 from human blood. 1) Crude plasma was passed over a Q sepharose column to remove albumin and other contaminants. 2) Material that did not bind Q sepharose was concentrated by ammonium sulfate precipitation. 3) Concentrated material was passed over an antibody column containing an irrelevant monoclonal antibody against MOPC. 4) MOPC column flow through was passed over a column containing the CTLA-4 specific antibody AS32. Bound material was then eluted and collected.
Tector et al. BMC Immunology 2009 10:51 doi:10.1186/1471-2172-10-51