Additional file 4.γ-Secretase-inhibitor (GSI) titration of OP9-cultured DN3 cells. Sorted SLY1+/+, SLY1-/- and SLY1Δ/Δ DN3 thymocytes were cultured on OP9 DL-1 or OP9 GFP cells for six days before analysis. Then, CD4- and CD8-expression was assessed by FACS (left panel). The expansion rate based on initial seeding numbers was determined (right panel). *p <0.01; **p <0.001; *** p <0.0001. Zipped folder containing EPS file. Format: ZIP Size: 1021KB Download file Reis et al. BMC Immunology 2009 10:38 doi:10.1186/1471-2172-10-38 |