Table 2

PCR primers and probes

Genes

Sequences

Sizes (bp)

TLR-1

(F) 5' CAG TGT CTG GTA CAC GCA TGG T 3'

105

(P) 5' (FAM) CAC ATG CTT TGC CAT CCA AAA TTA G (TARMA) 3'

(R) 5' TTT CAA AAA CCG TGT CTG TTA AGA GA 3'

TLR-2

(F) 5' TGT GAA GAG TGA GTG GTG CAA GT 3'

78

(P) 5' (FAM) TGA ACT GGA CTT CTC CCA TTT CCG TCT TT (TARMA) 3'

(R) 5' ATG GCA GCA TCA TTG TTC TCA T 3'

TLR-3

(F) 5' CCT GGT TTG TTA ATT GGA TTA ACG A 3'

82

(P) 5' (FAM) CAT ACC AAC ATC CCT GAG CT (MGB) 3'

(R) 5' TGA GGT GGA GTG TTG CAA AGG 3'

TLR-4

(F) 5' CAC TCG ATG TCA TTC CAA AGT TAT TG 3'

88

(P) 5' (FAM) TAC TAA GTA ATG ACT GTC ATG AAA GCA GCA T (TARMA) 3'

(R) 5' AGA GTG CCC CCT TTA AAC AAA TT 3'

TLR-5

(F) 5' TGC CTT GAA GCC TTC AGT TAT G 3'

77

(P) 5' (FAM) CCA GGG CAG GTG CTT ATC TGA CCT TAA CA (TARMA) 3'

(R) 5' CCA ACC ACC ACC ATG ATG AG 3'

TLR-6

(F) 5' GAA GAA GAA CAA CCC TTT AGG ATA GC 3'

88

(P) 5' (FAM) TGC AAC ATC ATG ACC AAA GAC AAA GAA CCT (TARMA) 3'

(R) 5' AGG CAA ACA AAA TGG AAG CTT 3'

TLR-7

(F) 5' TTA CCT GGA TGG AAA CCA GCT AC 3'

72

(P) 5' (FAM) AGA TAC CGC AGG GCC TCC CGC (TARMA) 3'

(R) 5' TCA AGG CTG AGA AGC TGT AAG CTA G 3'

TLR-8

(F) 5' AGC GGA TCT GTA AGA GCT CCA TC 3'

107

(P) 5' (FAM) CCT GAC AAC CCG AAG GCA GAA GGC (TARMA) 3'

(R) 5' CCG TGA ATC ATT TTC AGT CAA GAC 3'

TLR-9

(F) 5' GCA GTC AAT GGC TCC CAG TTC 3'

116

(P) 5' (FAM) CCC ACA ATA AGC T (MGB) 3'

(R) 5' GCG GTA GCT CCG TGA ATG AGT G 3'

TLR-10

(F) 5' TTA TGA CAG CAG AGG GTG ATG C 3'

152

(P) 5' (FAM) TTG ACC CCA GCC ACA ACG ACA CTG (TARMA) 3'

(R) 5' CTG GAG TTG AAA AAG GAG GTT ATA GG 3'

CCR-1

(F) 5' CCC AAT GGG AAT TCA CTC ACC A 3'

200

(P) 5' (FAM) CCT GCA GCC TTC ACT TTC CTC ACG AAA (TARMA) 3'

(R) 5' CAA ACG GAC AGC TTT GGA TT 3'

CCR-3

(F) 5' CAC AGC AGA GCC GGA ACT C 3'

75

(P) 5' (FAM) TGT GTT TTA GGT CAG ATG CAG AAA AT (TARMA) 3'

(R) 5' CTC CTT GGT CCT TCC TCT TTA GG 3'

CCR-5

(F) 5' ACT GCA AAA GGC TGA AGA GC 3'

204

(P) 5' (FAM) ACT GAC ATC TAC CTG CTC AAC CTG GCC A (TARMA) 3'

(R) 5' CGA TTG TCA GGA GGA TGA TG 3'

CCR-7

(F) 5' ACG GAC GAT TAC ATC GGA GA 3'

195

(P) 5' (FAM) ACA CGA CCA GCC CAT TGC CCA GT (TARMA) 3'

(R) 5' GGT CAT GGT CTT GAG CCT CT 3'

FasL

(F) 5' ACC TCA AGG GGG ACT GTC TT 3'

170

(P) 5' (FAM) ACA TGG TTG TGA CCT GAG GAT TTA AGG GAT GG (TARMA) 3'

(R) 5' TTA GTT TCA CCG ATG GCT CA 3'

TRAIL

(F) 5' CCC CTG CTG GCA AGT CAA 3'

68

(P) 5' (FAM) TGG CAA CTC CGT CAG CTC GTT (TARMA) 3'

(R) 5' CCT CAG AGG TTC TCA AAA TCA TCT T 3'

F, forward primers; R, reverse primers; P, Taqman probes

TLR, toll-like receptor; CCR, chemokine receptor; FasL, Fas ligand; TRAIL, Tumor necrosis factor (TNF)-related apoptosis-inducing ligand

Law et al. BMC Immunology 2009 10:35   doi:10.1186/1471-2172-10-35