24:26 increases the interaction of CTLA-4 with PP2A. Stably transfected Jurkat T cells were induced overnight with doxycycline (1 μg/ml) to express A) WT CTLA-4, B) KLESS CTLA-4, C) Y165F CTLA-4, D) PRO- CTLA-4, E) Y182F CTLA-4 or F) Y165F/Y182F CTLA-4. The cells were stimulated with or without 24:26 (100 μg/ml) for 60 minutes at 37°C. Cells were washed then lysed in standard lysis buffer containing Triton X-100 (1%). Lysates were used for immunoprecipitation of PP2AA, and subsequently blotted for CTLA-4, PP2AA and PP2AC. All data is representative of at least two independent experiments.
Teft et al. BMC Immunology 2009 10:23 doi:10.1186/1471-2172-10-23