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Open Access Research article

Effect of thyroid hormone concentration on the transcriptional response underlying induced metamorphosis in the Mexican axolotl (Ambystoma)

Robert B Page12, Stephen R Voss12*, Amy K Samuels12, Jeramiah J Smith12, Srikrishna Putta12 and Christopher K Beachy34

  • * Corresponding author: Stephen R Voss srvoss@uky.edu

  • † Equal contributors

Author Affiliations

1 Department of Biology, University of Kentucky, Lexington Kentucky, 40506 USA

2 Spinal Cord and Brain Injury Research Center, University of Kentucky, 40506 USA

3 Department of Biology, Minot State University, Minot, North Dakota, 58707 USA

4 Amphibian Growth Project, Minot State University, Minot, North Dakota, 58707 USA

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BMC Genomics 2008, 9:78  doi:10.1186/1471-2164-9-78

Published: 11 February 2008

Abstract

Background

Thyroid hormones (TH) induce gene expression programs that orchestrate amphibian metamorphosis. In contrast to anurans, many salamanders do not undergo metamorphosis in nature. However, they can be induced to undergo metamorphosis via exposure to thyroxine (T4). We induced metamorphosis in juvenile Mexican axolotls (Ambystoma mexicanum) using 5 and 50 nM T4, collected epidermal tissue from the head at four time points (Days 0, 2, 12, 28), and used microarray analysis to quantify mRNA abundances.

Results

Individuals reared in the higher T4 concentration initiated morphological and transcriptional changes earlier and completed metamorphosis by Day 28. In contrast, initiation of metamorphosis was delayed in the lower T4 concentration and none of the individuals completed metamorphosis by Day 28. We identified 402 genes that were statistically differentially expressed by ≥ two-fold between T4 treatments at one or more non-Day 0 sampling times. To complement this analysis, we used linear and quadratic regression to identify 542 and 709 genes that were differentially expressed by ≥ two-fold in the 5 and 50 nM T4 treatments, respectively.

Conclusion

We found that T4 concentration affected the timing of gene expression and the shape of temporal gene expression profiles. However, essentially all of the identified genes were similarly affected by 5 and 50 nM T4. We discuss genes and biological processes that appear to be common to salamander and anuran metamorphosis, and also highlight clear transcriptional differences. Our results show that gene expression in axolotls is diverse and precise, and that axolotls provide new insights about amphibian metamorphosis.