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Transcriptomic dissection of tongue squamous cell carcinoma

Hui Ye1, Tianwei Yu2, Stephane Temam34, Barry L Ziober5, Jianguang Wang6, Joel L Schwartz78, Li Mao3, David T Wong9 and Xiaofeng Zhou1108*

  • * Corresponding author: Xiaofeng Zhou

  • † Equal contributors

Author Affiliations

1 Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA

2 Department of Biostatistics, Rollins School of Public Health, Emory University, Atlanta, GA, USA

3 Department of Thoracic/Head and Neck Medical Oncology, University of Texas, MD Anderson Cancer Center, Houston, TX, USA

4 Department of Head and Neck Surgery, Institut Gustave-Roussy, Villejuif, France

5 Department of Otorhinolaryngology – Head and Neck Surgery, University of Pennsylvania Health System, Philadelphia, PA, USA

6 Department of Oral & Maxillofacial Surgery, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, China

7 Oral Medicine & Diagnostic Sciences, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA

8 Graduate College, UIC Cancer Center, University of Illinois at Chicago, Chicago, IL, USA

9 Dental Research Institute, School of Dentistry, Jonsson Comprehensive Cancer Center, University of California at Los Angeles, Los Angeles, CA, USA

10 Guanghua School & Research Institute of Stomatology, Sun Yat-sen University, Guangzhou, China

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BMC Genomics 2008, 9:69  doi:10.1186/1471-2164-9-69

Published: 6 February 2008



The head and neck/oral squamous cell carcinoma (HNOSCC) is a diverse group of cancers, which develop from many different anatomic sites and are associated with different risk factors and genetic characteristics. The oral tongue squamous cell carcinoma (OTSCC) is one of the most common types of HNOSCC. It is significantly more aggressive than other forms of HNOSCC, in terms of local invasion and spread. In this study, we aim to identify specific transcriptomic signatures that associated with OTSCC.


Genome-wide transcriptomic profiles were obtained for 53 primary OTSCCs and 22 matching normal tissues. Genes that exhibit statistically significant differences in expression between OTSCCs and normal were identified. These include up-regulated genes (MMP1, MMP10, MMP3, MMP12, PTHLH, INHBA, LAMC2, IL8, KRT17, COL1A2, IFI6, ISG15, PLAU, GREM1, MMP9, IFI44, CXCL1), and down-regulated genes (KRT4, MAL, CRNN, SCEL, CRISP3, SPINK5, CLCA4, ADH1B, P11, TGM3, RHCG, PPP1R3C, CEACAM7, HPGD, CFD, ABCA8, CLU, CYP3A5). The expressional difference of IL8 and MMP9 were further validated by real-time quantitative RT-PCR and immunohistochemistry. The Gene Ontology analysis suggested a number of altered biological processes in OTSCCs, including enhancements in phosphate transport, collagen catabolism, I-kappaB kinase/NF-kappaB signaling cascade, extracellular matrix organization and biogenesis, chemotaxis, as well as suppressions of superoxide release, hydrogen peroxide metabolism, cellular response to hydrogen peroxide, keratinization, and keratinocyte differentiation in OTSCCs.


In summary, our study provided a transcriptomic signature for OTSCC that may lead to a diagnosis or screen tool and provide the foundation for further functional validation of these specific candidate genes for OTSCC.