Sequence pattern of LPXTG-anchoring cell wall proteins and identification of Ssu05_1371. a) Structure of the primary sequence of cell-wall anchored proteins. They have the following elements: a signal peptide (SP) at the N-terminus and, at the C-terminus, the consensus sequence LPXTG for its recognition by a sortase, an enzyme that cleaves between T and G residues and binds the mature protein to the peptidoglycan layers of the cell wall. Following the LPXTG sequence, there is a hydrophobic region for transmembrane spanning (TM) of the immature form, and after, a short positively charged tail (+). b) Protein Ssu05_1371 shows the typical structure of a cell-wall protein. In red bold, sequence coverage by identified peptides by proteomics is shown (see Additional File 1).
Rodríguez-Ortega et al. BMC Genomics 2008 9:588 doi:10.1186/1471-2164-9-588