BMC Genomics

official impact factor 4.21

Open Access Highly Access Research article

Analysis of the Pythium ultimum transcriptome using Sanger and Pyrosequencing approaches

Foo Cheung1, Joe Win2, Jillian M Lang3, John Hamilton4, Hue Vuong1, Jan E Leach3, Sophien Kamoun2, C André Lévesque5, Ned Tisserat3 and C Robin Buell4*

Author Affiliations

1 The J. Craig Venter Institute, 9704 Medical Center Dr, Rockville, MD 20850 USA

2 The Sainsbury Laboratory, Colney Lane, Norwich, NR4 7UH, UK

3 Colorado State University, Department of Bioagricultural Sciences and Pest Management, C129 Plant Sciences, Ft. Collins CO 80523, USA

4 Michigan State University, Department of Plant Biology, East Lansing MI 48824, USA

5 Agriculture and Agri-Food Canada, Ottawa, ON, K1A 0C6, Canada

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BMC Genomics 2008, 9:542 doi:10.1186/1471-2164-9-542

Published: 15 November 2008

Additional files

Additional file 1:

Hybrid assembly singleton reads derived solely from Sanger reads.

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Additional file 2:

Hybrid assemblies derived solely from Sanger reads.

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Additional file 3:

Top 50 largest assemblies from the hybrid assembly.

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Additional file 4:

Top 50 largest assemblies from 454-pyrosequencing only assembly.

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Additional file 5:

Top 50 largest assemblies from Sanger only sequence assembly.

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Additional file 6:

Alignments of P. ultimum sequences to effectors and Crinkler proteins.

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Additional file 7:

Assemblies with similarity to protease inhibitors and elicitins.

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Additional file 8:

Chlamydomonasflagellar proteins identified in the hybrid EST assembly.

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Additional file 9:

Simple Sequence Repeats identified in the hybrid assembly.

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