Table 2

Expected and observed allele sizes of conserved chicken-Charadriiformes microsatellite markers

Locus

Exp. allele size (bp)*

Observed allele size (bp)


Charadri

Lari

Scolopaci


KPL

OYS

AVO

GSH

WAU

CPR

BSK

GBT

RUF

RNP

GSN

DUN


a) standard primer design

BmaTATC371

200

144, 152, 156

144, 146

149, 153, 157, 161, 165

152

158, 171, 173, 176, 180, 181, 190

173, 185

178, 182

173, 177, 185

140

136

failed

136

Cmms 9

252

241

241

241

245

252, 256, 258, 262

252

255

250

246

244

failed

243

Cmms 26

313

320

318

319

339

304, 308, 310

320, 327, 329, 338

304

310

316, 324

324, 326

326

312

Mopl 6

281

290, 292, 294

274

274

273

278

failed

283

267

301, 303, 305

283, 287, 291, 303

277, 293, 295

284, 288, 290, 292

Mopl 18

141

139

137

137

152

136

129

136

128

137

failed

136, 140

136

Mopl 21

230

315, 321

317

321

320

315, 322

322

322

328, 330, 334

314, 320

316, 318

322

309, 311, 313

LarsZAP26/K32

125

100, 102

98

97

94

106, 108, 133

failed

120

122, 124, 126, 134

106

99

105

106, 108

b) consensus primer design

54F2

207

215, 217

206, 208

216

189

220

217, 221

228

219

212

212

218, 226

212

Apy07

191

146

146

144

146

170, 190, 194, 198, 202, 206

157

154

154

145

145

145

145

BmaAGGT503

255

249

248

249

247

251, 252, 253, 254

255

249

249

257

258

263

256

BmaTATC453

294

239

239

239

239

239

236

239

239, 241

239

239

237, 239, 241

239

BmaTGAA523

120

71

73

73

74

77

74

78

78

71

73

74

73

Calex-01

221

201, 209, 211, 213, 221, 227

197

200

197

201, 203

224

201

233, 235, 239, 244

207

197, 201

211

207

Calex-05

197

179, 181, 183

173

171

168

173

173, 175

173

177, 179

173

171, 173

171

173, 175

Cmms3

146

95

101

95

97

102, 106, 108, 110

93, 95, 97, 99, 101, 103

105

108

95

95

95

95

SNIPE B2

212

170, 172

183

failed

172

164

165

164

165

176, 180, 184, 196, 200

164, 168, 172, 184, 192, 196

188, 196, 200, 204, 208, 212

188

Mopl 15

170

174, 182

160

failed

161

182

178

172, 182

178

172

172

173

172

Mopl 22

404

394, 404

388

404

394

402

389

406

393

401

404

394, 404

412

Mopl 26

195

188, 190, 194, 200, 212

191

192, 194, 195

191

192, 193, 194

189, 199

188

185

184

174

184

185

Pgt 83

151

132

147

147

147

142

147

142

142

149

142, 144, 161

141, 143

142, 150, 156, 160

Rbg 18

270

259, 263, 265, 267, 269, 275, 277

262, 264

261, 265

268

260, 266, 272

Failed

268, 271

266

259, 261

265, 267

259

256, 260

Rbg 27

194

184

184

186

182

182

182

186, 188

182, 190, 196

184

186

186, 188

184

Rbg 29

129

127

125

125

127

116, 118, 126, 132, 136, 146

118, 120, 122

127

122, 132, 134, 142

127

127

120

failed


The markers were tested in four unrelated individuals from a single population of each of 12 species of Charadriiformes.

*expected product size in source species based on the sequence of the originally cloned allele.

KPL Kentish plover (Charadrius alexandrinus), OYS oystercatcher (Haematopus ostralegus), AVO avocet (Recurvirostra avosetta), GSH greater sheathbill (Chionis alba), WAU whiskered auklet (Aethia pygmaea), CPR collared pratincole (Glareola pratincola), BSK brown skua (Catharacta lonnbergi), GBT gull-billed tern (Gelochelidon nilotica), RUF ruff (Philomachus pugnax), RNP red-necked phalarope (Phalaropus lobatus), GSN great snipe (Gallinago media), DUN dunlin (Calidris alpina)

K├╝pper et al. BMC Genomics 2008 9:502   doi:10.1186/1471-2164-9-502

Open Data