Overview of the strategy. Viral particles are separated from host contaminants using centrifugation and filtration. Viral particles are treated with DNAse I to remove contaminated nucleic acids. Random priming is used to generate 500–1000 bp amplicons which are size-selected and cloned. Colonies are picked and sequenced. Sequence is trimmed and assembled. Contigs are closed using sequence-specific primers.
Djikeng et al. BMC Genomics 2008 9:5 doi:10.1186/1471-2164-9-5