Figure 7.

Effect of sequence length on coverage. Modified EST sets were generated from RefSeq sequences by truncating the sequences from the 5' end to a various extent. cDNA-AFLP sequential digestion protocol was simulated using the optimal combinations of two marking and three releasing enzymes. Squares connected by a dotted line: mouse; triangles connected by a dashed line: human; circles connected by a filled line: Arabidopsis.