Table 3

Validation of microarray-based mRNA levels by qRT-PCR

Microarray

qRT-PCR


Gene

Accession Number

B16 48 h RA vs. control

melan-a vs. B16

B16 48 h RA vs. control

melan-a vs. B16


BRCA2

NM_009765

0.30

0.36

0.301

0.0938

CDC2A

NM_007659

0.40

0.22

0.298

0.0311

TOP2A

NM_011623

0.50

0.11

0.226

0.00668

TINAGL

NM_023476

3.70

4.00

1.81

5.64

PLAT

NM_008872

2.10

3.33

3.15

1.43*

DHRS3

NM_011303

4.60

2.70

4.89

133

FGF1

NM_010197

2.20

2.27

3.53

2.35


All qRT-PCR values were significant by Bonferroni-corrected (n = 14) T-test with p < 0.01 with the exception of starred (*) value.

Estler et al. BMC Genomics 2008 9:478   doi:10.1186/1471-2164-9-478

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