Open Access Highly Accessed Research article

Analysis of wheat SAGE tags reveals evidence for widespread antisense transcription

Rebecca L Poole1*, Gary LA Barker1, Kay Werner1, Gaia F Biggi2, Jane Coghill3, J George Gibbings4, Simon Berry5, Jim M Dunwell4 and Keith J Edwards1

1 School of Biological Sciences, University of Bristol, Bristol, UK

2 School of Biological Sciences, University of Southampton, Southampton, UK

3 Transcriptomics Facility, School of Biological Sciences, University of Bristol, Bristol, UK

4 School of Biological Sciences, University of Reading, Reading, Berkshire, UK

5 Nickerson-Advanta, Station Road, Docking, Norfolk, UK

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BMC Genomics 2008, 9:475 doi:10.1186/1471-2164-9-475

Published: 10 October 2008

Additional files

Additional File 1:

Zipped folder containing txt files. PERL scripts used for SAGE data annotations and analysis

Format: ZIP Size: 19.5MB Download file

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Additional File 2:

Complete list of annotated SAGE tags (with count ≥2) and differential expression analysis.

Format: XLS Size: 4.6MB Download file

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Additional File 3:

Annotated list of singleton SAGE tags

Format: XLS Size: 16.6MB Download file

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Additional File 4:

Zip folder containing XLS and Word file. Data and discussion about the 50 most abundant sense UniGenes.

Format: ZIP Size: 66KB Download file

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Additional File 5:

27 UniGenes investigated for evidence of alternative polyadenylation.

Format: XLS Size: 63KB Download file

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Additional File 6:

50 most abundant antisense UniGenes.

Format: XLS Size: 129KB Download file

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Additional File 7:

Pin array oligo sequences.

Format: XLS Size: 78KB Download file

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