Table 2 |
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|
Transcript level comparison of P. aeruginosa genes between real-time PCR and microarray analyses |
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|
Gene |
amRNA level change with microarray |
bmRNA level change with real-time PCR |
Forward primer sequence (5'-3') |
Reverse primer sequence (5'-3') |
||
|
|
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|
Fold change |
Fold change |
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|
|
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|
20 min |
60 min |
20 min |
60 min |
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|
|
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|
PA4243 |
2.914 |
6.799 |
3.605 (± 1.55) |
7.378 (± 0.7) |
ATGGCTAAGCAAGGTGCTCTCTCT |
ACGATGATCGCCAGGAACAGGAAA |
|
PA2193 |
-2.268 |
-1.967 (± 1.06) |
TGAACGTCAACACGATATCCAGCC |
ATTGAGCACGTTGAGCACGGTCT |
||
|
PA1173 |
-3.077 |
-3.03 (± 5.64) |
ATCGACAAGGACAGCAACAAGTGC |
GTCCATGTAGTGGGTGATGCTGAT |
||
|
PA3049 |
-6.25 |
-25.907 |
-10.196 (± 0.14) |
-48.503 (± 0.35) |
TCGTGATCTTTGTCCGTTCACCCA |
CGTGCTGGAGTTGATTGAGACGTT |
|
PA3724 |
-2.762 |
-10.256 |
-5.856 (± 0.21) |
-14.928 (± 1.27) |
TCATCACCGTCGACATGAACAGCA |
AGTCCCGGTACAGTTTGAACACCA |
|
PA3622 |
-2.653 |
-2.967 |
-1.954 (± 1.95) |
-5.924 (± 1.60) |
TGACCACGATGATGAAGTGCTCCT |
TTGGAAGAGAAGGAAGTGGTGGCT |
|
cPA3001 |
1.00 |
1.00 |
1.00 |
1.00 |
GCACCATCACCATCGACGAAGAAA |
TCTTGATGCCGTACTGGGTGTAGT |
|
|
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|
The real time PCR results are the mean of three biological replicates with three technical replicates for each gene. The microarray results are the mean of four replicates of each gene. aThe microarray results are the mean of four replicates of each gene. bThe real time PCR results are the mean of three biological replicates with three technical replicates for each gene. cInternal control: glyceraldehyde-3-phosphate dehydrogenase |
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|
Nde et al. BMC Genomics 2008 9:473 doi:10.1186/1471-2164-9-473 |
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