Table 2 |
||||||
|
Percentage nucleotide (nts) and amino acid (aa) identity amongst domains of the mycolactone PKS from pMUM001 and pMUM002 |
||||||
|
pMUM001 |
pMUM002 |
Interspecies |
||||
|
|
||||||
|
Domain (nts/aa) |
nts |
aa |
nts |
aa |
nts |
aa |
|
|
||||||
|
Ketosynthase-load (1149/383) |
100 |
100 |
100 |
100 |
99.6 |
98.7 |
|
|
||||||
|
Ketosynthase (1260/419) |
98.9 |
97.3 |
99.1 |
97.8 |
98.6 |
96.7 |
|
|
||||||
|
Acyltransferase-I (1056/352) |
100 |
100 |
100 |
100 |
99.8 |
99.7 |
|
|
||||||
|
Acyltransferase-II (999/333) |
100 |
100 |
100 |
100 |
99.2 |
99.1 |
|
|
||||||
|
Acyltransferase-III (1056/352) |
99.9 |
100 |
100 |
100 |
99.8 |
99.7 |
|
|
||||||
|
Dehydratase (465/155) |
98.7 |
96.1 |
99.3 |
99.3 |
98.3 |
98.7 |
|
|
||||||
|
Enoylreductase (948/316) |
100 |
100 |
100 |
100 |
100 |
100 |
|
|
||||||
|
Ketoreductase A (528/176) |
100 |
100 |
100 |
100 |
100 |
100 |
|
|
||||||
|
Ketoreductase B (540/180) |
99.6 |
98.9 |
99.4 |
98.3 |
98.7 |
97.2 |
|
|
||||||
|
Acyl carrier protein-I (210/70) |
95.7 |
95.7 |
98.6 |
100 |
95.2 |
95.7 |
|
|
||||||
|
Acyl carrier protein-II (210/70) |
100 |
100 |
99.5 |
98.6 |
99.1 |
97.1 |
|
|
||||||
|
Acyl carrier protein-III (210/70) |
98.6 |
98.6 |
97.6 |
95.7 |
96.2 |
94.3 |
|
|
||||||
|
Chromosomal |
99.6* |
99.4 |
||||
|
|
||||||
|
Plasmid (other) |
99.7 |
99.3 |
||||
|
|
||||||
|
* Comparisons of plasmid and chromosomal sequences are taken from mulit-locus sequence analysis of concatenated DNA sequences of 3210 nts and 1070 nts respectively [6]. |
||||||
|
Pidot et al. BMC Genomics 2008 9:462 doi:10.1186/1471-2164-9-462 |
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