Research article
Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactones
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* Corresponding author: Timothy P Stinear tim.stinear@med.monash.edu.au
1 Department of Microbiology, Monash University, Clayton, 3800, Australia
2 Department of Biochemistry, University of Cambridge, Cambridge, CB21EW, UK,
3 Victorian Bioinformatics Consortium, Monash University, Clayton, 3800, Australia
4 Australian Genome Research Facility, University of Queensland, St Lucia, 4072, Australia
BMC Genomics 2008, 9:462 doi:10.1186/1471-2164-9-462
Published: 7 October 2008Additional files
Additional file 1:
Shared DNA between pMUM plasmids (A) Dot plots showing a two-way comparison of DNA identity over the length of the non-PKS region of pMUM001, pMUM002 and pMUM003. (B) Venn diagram showing the shared and unique CDS amongst the three pMUM plasmids.
Format: TIFF Size: 2.5MB Download file
Additional file 2:
Description of other features of pMUM001 and pMUM002, not directly associated with mycolactone synthesis.
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Additional file 2:
Table summary of the 96 predicted CDS in pMUM002.
Format: RTF Size: 735KB Download file
Additional file 4:
ClustalW alignment. Alignment of the derived amino acid sequences for the AT domains from the load modules of MlsA1 and MlsB from M. liflandii 128FXT, showing the AT domain from MlsB has a sequence consistent with methylmalonate specificity. Boxed sequences are residues known to be critical for AT substrate specificity.
Format: TIFF Size: 1.9MB Download file