Table 1

ENU-induced mutation frequencies

Genotype/Dosea

Msh6-/-3 × 25

Msh6-/-3 × 30

Msh6-/-3 × 35

Total

WT Control 3 × 40b


# Males injected

6

8

8

24

10

# Fertile malesc

N/D

5

1

6

# Pups for screening

16

291

3

310

362

# Bases screened

3.6 × 106

69.7 × 106

0.7 × 106

74.0 × 106

37.3 × 106

# Mutations

- nonsense

0

4

0

4

0

- missense

4

59

1

64

17

- silent

0

16

0

16

7

- non-coding

0

5

0

5

6

- total

4

84

1

89

30

Mutation Frequencies (bp-1)

1.12 × 10-6

1.21 × 10-6

1.38 × 10-6

8.05 × 10-7

Mutation Rates (bp)

1 in 8.93 × 105

1 in 8.29 × 105

1 in 7.22 × 105

1 in 12.4 × 105


aDose is indicated as mg of ENU per kg bodyweight (three weekly injections)

bWT (Wild Type) control 3 × 40 mg/kg measurements were adapted from [5] and represent the highest ENU-induced mutation frequency in rats

cFertility is measured by the presence of at least one litter within 10 weeks after the last injection.

Sterility was confirmed by histological examination (N/D = not determined)

van Boxtel et al. BMC Genomics 2008 9:460   doi:10.1186/1471-2164-9-460

Open Data