Probability of gene knockouts in Msh6-deficient and wild type rats. The chance to retrieve a knockout for any given gene and the total number of genes that will be knocked out when all genes would be screened for mutations is plotted as a function of the number of mutant F1 animals for wild type (WT, 40 mg ENU/kg, black line) and MMR-deficient (Msh6-/-, 30 mg ENU/kg, red line) rats. The red dashed lines show the number of animals needed to knockout 95% of all genes and any given gene with 95% chance. The use of a Msh6-deficient background reduces the number of animals ~2.5-fold.
van Boxtel et al. BMC Genomics 2008 9:460 doi:10.1186/1471-2164-9-460