Heat stress-responsive transcriptome analysis in heat susceptible and tolerant wheat (Triticum aestivum L.) by using Wheat Genome Array
- Equal contributors
1 Department of Plant Genetics & Breeding and State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing100193, PR China
2 Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA) and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing100193, PR China
BMC Genomics 2008, 9:432 doi:10.1186/1471-2164-9-432Published: 22 September 2008
Wheat is a major crop in the world, and the high temperature stress can reduce the yield of wheat by as much as 15%. The molecular changes in response to heat stress are poorly understood. Using GeneChip® Wheat Genome Array, we analyzed genome-wide gene expression profiles in the leaves of two wheat genotypes, namely, heat susceptible 'Chinese Spring' (CS) and heat tolerant 'TAM107' (TAM).
A total of 6560 (~10.7%) probe sets displayed 2-fold or more changes in expression in at least one heat treatment (
The differences in heat tolerance in different wheat genotypes may be associated with multiple processes and mechanisms involving HSPs, transcription factors, and other stress related genes. Heat acclimation has little effects on gene expression under prolonged treatments but affects gene expression in wheat under short-term heat stress. The heat stress responsive genes identified in this study will facilitate our understanding of molecular basis for heat tolerance in different wheat genotypes and future improvement of heat tolerance in wheat and other cereals.