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Open Access Highly Accessed Research article

Prediction of Sinorhizobium meliloti sRNA genes and experimental detection in strain 2011

Claudio Valverde1*, Jonathan Livny2, Jan-Philip Schlüter34, Jan Reinkensmeier5, Anke Becker34 and Gustavo Parisi6

Author Affiliations

1 Programa Interacciones Biológicas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, Bernal, Buenos Aires, B1876BXD, Argentina

2 Channing Laboratories, Brigham and Women's Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA

3 Institute for Genome Research and Systems Biology, Center for Biotechnology (CeBiTec), Bielefeld University, 33594 Bielefeld, Germany

4 Institute of Biology III, Faculty of Biology, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany

5 Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany

6 Grupo de Bioinformática Estructural, Centro de Estudios e Investigaciones, Universidad Nacional de Quilmes, Roque Saénz Peña 352, Bernal, Buenos Aires, B1876BXD, Argentina

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BMC Genomics 2008, 9:416  doi:10.1186/1471-2164-9-416

Published: 16 September 2008

Abstract

Background

Small non-coding RNAs (sRNAs) have emerged as ubiquitous regulatory elements in bacteria and other life domains. However, few sRNAs have been identified outside several well-studied species of gamma-proteobacteria and thus relatively little is known about the role of RNA-mediated regulation in most other bacterial genera. Here we have conducted a computational prediction of putative sRNA genes in intergenic regions (IgRs) of the symbiotic α-proteobacterium S. meliloti 1021 and experimentally confirmed the expression of dozens of these candidate loci in the closely related strain S. meliloti 2011.

Results

Our first sRNA candidate compilation was based mainly on the output of the sRNAPredictHT algorithm. A thorough manual sequence analysis of the curated list rendered an initial set of 18 IgRs of interest, from which 14 candidates were detected in strain 2011 by Northern blot and/or microarray analysis. Interestingly, the intracellular transcript levels varied in response to various stress conditions. We developed an alternative computational method to more sensitively predict sRNA-encoding genes and score these predicted genes based on several features to allow identification of the strongest candidates. With this novel strategy, we predicted 60 chromosomal independent transcriptional units that, according to our annotation, represent strong candidates for sRNA-encoding genes, including most of the sRNAs experimentally verified in this work and in two other contemporary studies. Additionally, we predicted numerous candidate sRNA genes encoded in megaplasmids pSymA and pSymB. A significant proportion of the chromosomal- and megaplasmid-borne putative sRNA genes were validated by microarray analysis in strain 2011.

Conclusion

Our data extend the number of experimentally detected S. meliloti sRNAs and significantly expand the list of putative sRNA-encoding IgRs in this and closely related α-proteobacteria. In addition, we have developed a computational method that proved useful to predict sRNA-encoding genes in S. meliloti. We anticipate that this predictive approach can be flexibly implemented in many other bacterial species.