Table 8

Differentially expressed genes encoding transcription-associated proteins in artesunate-treated cells

Gene ID

Description

Pfam

annotation

ART 90 min

ART 3 h

HS

CQ


PF11_0053

PfSNF2L

TAP clustering

Nucleosome remodeling::SNF2L

0,82

PF11_0264

DNA-dependent RNA polymerase

TAP clustering

MITOCHONDRIAL RNA POLYMERASE

1,02

PFL0560c

minichromosome maintenance protein, putative

TAP clustering

Minichromosome maintenance protein

0,85

PFE0090w

hypothetical protein, conserved

TAP clustering

Histone trancription regulator

0,82

PF11_0297

hypothetical protein

TAP clustering

CCR4-NOT complex::NOT2

0,86

+

PFE1245w

hypothetical protein, conserved

PF00642

Zinc finger C-x8-C-x5-C-x3-H type (and similar)(PF00642)

1,28

0,91

+

+

PF14_0236

hypothetical protein

PF00642

Zinc finger C-x8-C-x5-C-x3-H type (and similar)(PF00642)

0,89

+

PFC0680w

hypothetical protein, conserved

PF00642

Zinc finger C-x8-C-x5-C-x3-H type (and similar)(PF00642)

1,55

+

PF10_0186

hypothetical protein

PF00642

Zinc finger C-x8-C-x5-C-x3-H type (and similar)(PF00642)

0,98

MAL13P1.122

hypothetical protein, conserved

PF00856

SET domain (PF00856)

0,81

PFI0470w

FHA domain protein, putative

PF00097

C3HC4 type (RING finger) (PF00097)

0,89

1,05

PF10_0046

hypothetical protein

PF00097

C3HC4 type (RING finger) (PF00097)

1,17

PFF0165c

hypothetical protein, conserved

PF00097

C3HC4 type (RING finger) (PF00097)

0,94

PFL0440c

hypothetical protein, conserved

PF00097

C3HC4 type (RING finger) (PF00097)

0,95

PF11_0315

hypothetical protein

TAP clustering

APICOPLAST RNA beta, beta, & beta subunits

1,64

PFL0145c

high mobility group protein

PF00505

HMG (high mobility group) box (PF00505)

-0,97

MAL13P1.76

TFIIH basal transcription factor subunit

TAP clustering

TFIIH::p44

-0,83

PF14_0718

hypothetical protein, conserved

TAP clustering

RNA polymerase II-associated factor SOH1

-0,86


Gene ID: identifier as found in PlasmoDB.

Art columns: log ratios of gene expression under artesunate exposure at 90 minutes and 3 hours.

HS and CQ columns: over (+) expression.

Results were compared with transcriptome modifications induced by heat-shock (HS) [12] and chloroquine (CQ) [8].

Genes encoding transcription-associated proteins (TAPs) were selected from the TAP clustering of Coulson et al. [17] and completed with an update of genes fitting the following HMM profiles defined by Coulson and colleagues [16] : PF00097, PF00642, PF00096, PF00856, PF00249, PF00439, PF00505, PF00628, PF00098, PF00808, PF01753, PF00313, PF00569, PF00643, PF08523, PF02146, PF03366.

Natalang et al. BMC Genomics 2008 9:388   doi:10.1186/1471-2164-9-388

Open Data