Table 3

I. scapularis differentially expressed genes after subolesin knockdown.

Clone IDa

Accession number, name and speciesb

Fold change (6 dpi)c

SD (6 dpi)d

Fold change (9 dpi)c

SD (9 dpi)d


LibPlateC1_wellB12

[Genbank:AAT92189] KUN-6 (Ixodes pacificus)

-1.1196

0.2519

-2.2057

0.3109

LibPlateC1_wellB6

[Genbank:AAZ29240] copper/zinc superoxide dismutase (Macrobrachium rosenbergii)

-2.7645

0.5455

-2.5136

0.2930

LibPlateC1_wellD9

[Genbank:AAY66901] histone 2B (Ixodes scapularis)

-1.5922

1.5332

-2.5298

0.9579

LibPlateC1_wellE6

[Genbank:AAY66498] putative secreted salivary WC peptide (Ixodes scapularis)

1.2320

1.4160

-5.2598

1.3686

LibPlateC1_wellF10

[Genbank:AAY66817] anticoagulant Salp9-like (Ixodes scapularis)

-2.0332

0.2959

-1.2037

0.1124

LibPlateC1_wellG12

[Genbank:AAZ29240] copper/zinc superoxide dismutase (Macrobrachium rosenbergii)

-1.6426

0.2527

-2.2118

0.4974

LibPlateC1_wellH10

No homology found

-1.8346

0.7216

-2.0350

1.0821

LibPlateC1_wellH4

No homology found

-1.6161

0.4640

-2.0602

0.5671

LibPlateC2_wellB11

[Genbank:AAY66498] putative secreted salivary WC peptide (Ixodes scapularis)

-1.8612

0.1952

-2.1981

0.1664

LibPlateC2_wellB12

No homology found

-1.1313

0.1588

-2.1133

0.2023

LibPlateC2_wellD5

No homology found

-1.6882

0.0965

-2.2689

0.2158

LibPlateC2_wellE3

[Genbank:AAY66498] putative secreted salivary WC peptide (Ixodes scapularis)

-1.0794

0.2033

-2.2673

0.2302

LibPlateC2_wellG1

[Genbank:AAM93633|AF483711_1] putative secreted protein (Ixodes scapularis)

-1.4409

0.2000

-3.5351

0.3250

LibPlateC3_wellB10

[Genbank:AAH67494] HIST1H3I protein (Homo sapiens)

-2.0111

0.2174

-2.3299

0.4323

LibPlateC3_wellB8

No homology found

-2.0438

1.5307

-1.3772

0.5537

LibPlateC3_wellC7

[Genbank:AAZ29240] copper/zinc superoxide dismutase (Macrobrachium rosenbergii)

-4.1699

0.1770

-3.1053

0.0638

LibPlateC3_wellD7

[Genbank:AAZ29240] copper/zinc superoxide dismutase (Macrobrachium rosenbergii)

-2.5487

0.1977

-2.1210

0.4374

LibPlateC3_wellE2

[Genbank:XP_624446.2] Predicted similar to ruby CG11427-PA isoform 2 (Apis mellifera)

-2.4606

1.6665

-1.0115

0.3122

LibPlateC3_wellE9

[Genbank:AAZ29240] copper/zinc superoxide dismutase (Macrobrachium rosenbergii)

-3.4248

0.3717

-2.0792

0.2988

LibPlateC3_wellF10

[Genbank:AAM93633|AF483711_1] putative secreted protein (Ixodes scapularis)

-1.3583

0.2449

-3.9731

0.1679

LibPlateC3_wellF11

[Genbank:AAM93633|AF483711_1] putative secreted protein (Ixodes scapularis)

-1.0215

0.1441

-2.9246

0.1172

LibPlateC3_wellG5

[Genbank:AAM93633|AF483711_1] putative secreted protein (Ixodes scapularis)

-1.1451

0.2311

-3.7711

0.1890

LibPlateC4_wellB1

No homology found

-1.7450

0.6400

-2.2268

0.3409

LibPlateC4_wellB10

[Genbank:AAQ01562] von Willebrand factor (Ixodes ricinus)

1.1202

0.3737

-3.9449

0.2800

LibPlateC4_wellB5

[Genbank:ABD83654] hemelipoglycoprotein precursor (Dermacentor variabilis)

-1.9958

0.5689

-3.5813

0.6757

LibPlateC4_wellB7

[Genbank:AAP84098] ML domain-containing protein (Ixodes ricinus)

-1.5703

0.3164

-4.5599

0.4105

LibPlateC4_wellB8

No homology found

-2.6551

0.3564

-3.5944

0.3325

LibPlateC4_wellC1

[Genbank:AAY66502] secreted salivary gland protein (Ixodes scapularis)

1.0009

0.2427

-2.2922

0.3518

LibPlateC4_wellC2

No homology found

-1.0286

0.0610

-2.0853

0.1338

LibPlateC4_wellC6

No homology found

-1.1217

0.1682

-2.7104

0.1235

LibPlateC4_wellD3

No homology found

1.6859

0.2075

2.6468

0.1954

LibPlateC4_wellD4

[Genbank:XP_974124] PREDICTED: similar to CG2972-PA (Tribolium castaneum)

-2.1335

0.2315

-2.6578

0.3642

LibPlateC4_wellF6

[Genbank:BAE53722] aspartic protease (Haemaphysalis longicornis)

-1.4881

0.2385

-4.8576

0.3690

LibPlateC4_wellG3

[Genbank:AAY66629] putative secreted salivary protein (Ixodes scapularis)

1.0601

0.1625

2.0049

0.1481

LibPlateC4_wellG4

[Genbank:XP_794044] Predicted similar to Endoplasmic reticulum-golgi intermediate

-2.2835

0.2324

-1.7916

0.5510

LibPlateC4_wellH4

[Genbank:AAY66660] putative salivary secreted protein (Ixodes scapularis)

-1.5502

0.2177

-5.1239

0.2056

LibPlateC4_wellH6

[Genbank:AAY66982] cyclophilin A (Ixodes scapularis)

-1.0545

0.0098

2.0388

0.2239

LibPlateR1_wellG3

No homology found

1.2792

0.2664

2.2118

0.1902

LibPlateR1_wellH7

[Genbank:AAY66764] putative secreted salivary protein (Ixodes scapularis)

1.2470

0.0447

-2.0598

0.0708

LibPlateR2_wellB2

[Genbank:AAY66713] putative secreted salivary protein (Ixodes scapularis)

1.4186

0.2490

2.2141

0.2145

LibPlateR2_wellF10

[Genbank:AAK97818|AF209915_1] 16 kDa salivary gland protein A (Ixodes scapularis)

-1.8100

0.4667

-2.4275

0.3690

LibPlateR2_wellG7

[Genbank:AAY66713] putative secreted salivary protein (Ixodes scapularis)

1.4101

0.1056

2.1495

0.1347

LibPlateR3_wellA7

No homology found

-1.0338

0.1570

2.0213

0.1464

LibPlateR4_wellA3

No homology found

-1.0990

1.1277

-3.4438

1.6338


aClone ID identifies the clone based on SSH Library Plate_well.

bName: This output only contains descriptions of the top blast hit (most significant alignment based on E value).

cFold change is of the global normalized ratio (log2(635/532)) of background-corrected means averaged between replicates (determined from valid spots only). Only genes down-regulated (negative values) and up-regulated (positive values) after subolesin knockdown by at least two fold at 6 or 9 dpi were considered.

dSD is the standard deviation determined from the normalized average log2 ratio (determined from valid spots only).

de la Fuente et al. BMC Genomics 2008 9:372   doi:10.1186/1471-2164-9-372

Open Data