Co-affinity purification assays. The supernatant of GI- and GII-expressing E. coli cell lysates were loaded onto Ni-NTA columns, washed, then loaded with purified recombinant subolesin and washed again before protein elution for SDS-PAGE and Western blot. The top panel shows subolesin bait detection after affinity purification on Ni-NTA columns in the presence (+) or absence (-) of His-tagged GI/GII protein extracts, demonstrating binding to His-tagged prey. The bottom panel shows detection of GI/GII His-prey after affinity purification on Ni-NTA columns.
de la Fuente et al. BMC Genomics 2008 9:372 doi:10.1186/1471-2164-9-372