Confirmation of the microarray results for potential Zap1 target genes. S1 nuclease protection assays were performed using RNA isolated from cells grown under the same conditions as microarray experiments E3 and E4. A) ZRT1 and CMD1 were used as positive and loading controls, respectively. Results with candidate genes from Table 1 (B), Table 2 (C) and Table 3 (D) are shown. The band intensities were quantified and normalized to CMD1 levels, and the fold increase in E3 and E4 conditions is reported. These data confirmed the microarray results for these genes.
Wu et al. BMC Genomics 2008 9:370 doi:10.1186/1471-2164-9-370