A. Overlaps between the datasets and percentage of genes with a RUNX1 binding site in their regulatory regions. The overlaps between the different platforms are represented with arrows. * indicates that the genes differentially expressed in at least one of the mouse datasets are considered for the following overlap. The number of differentially expressed genes (DEGs) containing a conserved RUNX1 binding site (with CBS) in their regulatory regions, as determined by the oPOSSUM program , over the number of analyzed genes is indicated for each dataset and overlap. The corresponding percentage is indicated in brackets. B. Luciferase assay for 5 RUNX1 binding sites corresponding to 3 differentially expressed genes. The transactivation activity of RUNX1 over these sites was measured as the fold change of the luciferase activity in the presence of the CBF complex compared to the endogenous activity of each construct. The standard errors of three independent replicates are shown. CASP3 was shown as a negative control as no binding site was found for this gene. The difference in expression for the three genes in each dataset is indicated in the table. 0 means no difference in expression, ↓ stands for down-regulated and ↑ stands for up-regulated.
Michaud et al. BMC Genomics 2008 9:363 doi:10.1186/1471-2164-9-363