Table 2

Primers and conditions for isoform-specific PCR analysis

PCR Primer Name

Primer Sequence

PCR Conditions


Prl6a1 forward

5'-GGTCATTTCCCAGATGCTGT-3'

1.5 mM MgCl2, 35 cycles, 60°C annealing

Prl6a1 common reverse

5'-GGGCTCCCTCCTTAGACACT-3'

Prl6a1 novel forward

5'-GCCTGAGAAGGCAAAGAAAA-3


Prl8a6 forward

5'-CTGCCATTCAAGTCTCACGA-3'

1.5 mM MgCl2, 30 cycles, 60°C annealing (Novel forward rxn – 35 cycles)

Prl8a6 common reverse

5'-CTCAAAAGCCAAGGAGATCG-3'

Prl8a6 novel forward

5'-GCTCGATTATATAGGAACAGGAA-3'


Prl8a8 forward

5'-GAAAAAGAATTGTGATT-3'

2.0 mM MgCl2, 35 cycles, 46°C annealing (Novel forward rxn – 1.5 mM MgCl2)

Prl8a8 common reverse

5'-TCAATAGTTATGTATTGAA-3'

Prl8a8 novel forward

5'-ATAACTCCGATGAAGC-3'


Prl8a1 reverse

5'-CCAAACAATCAACTGCCATG-3'

1.5 mM MgCl2, 30 cycles, 60°C annealing

Prl8a1 common forward

5'-AACCCCACTTCTGCACATTC-3'

Prl8a1 novel reverse

5'-CCCAAAAGATCGACCCATAA-3'


Prl7a1 reverse

5'-TCAGCCACATTTTCTGTTGC-3'

2.0 mM MgCl2, 28 cycles, 60°C annealing (Novel reverse rxn 32 cycles)

Prl7a1 common forward

5'-AAGGGACTGTTGGATCATGC-3'

Prl7a1 novel reverse

5'-TGGCATTCATGAGTGAGAAA-3'


Simmons et al. BMC Genomics 2008 9:352   doi:10.1186/1471-2164-9-352

Open Data