Cross-platform comparison between SYBR Green RT2 Profiler PCR Arrays and the three quantitative platforms. The concordance of fold-changes between the PCR Arrays and the three quantitative platforms was evaluated by regression analysis of fold differences in sample B compared to sample A. Data were normalized against POLR2A for RT2 Profiler PCR Arrays and TaqMan, and against beta-actin for StaRT-PCR. The sample B/sample A (B/A) fold changes (log2) for each gene common between RT2 Profiler PCR Arrays and another platform were subjected to bivariate analysis. The dashed line on each graph represents the ideal slope of 1.0. The solid lines show a linear regression fit.
Arikawa et al. BMC Genomics 2008 9:328 doi:10.1186/1471-2164-9-328