Inter-site comparison of RT2 Profiler PCR Arrays. Threshold cycle measurements and fold-change results have been obtained using the two MAQC reference RNAs analyzed on the Human Drug Metabolism RT2 Profiler PCR Array (APH-002) using different real-time PCR thermal cyclers at two different sites. Five replicate arrays were run for each sample at each site, and the average CT value for each assay is compared between the two sites in panels A and B. The results show a high correlation between CT values obtained from the two sites. Panel C shows the inter-site comparison of fold-change results obtained from these data, indicating a correlation coefficient of 0.976.
Arikawa et al. BMC Genomics 2008 9:328 doi:10.1186/1471-2164-9-328