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Open Access Highly Accessed Research article

Cross-platform comparison of SYBR® Green real-time PCR with TaqMan PCR, microarrays and other gene expression measurement technologies evaluated in the MicroArray Quality Control (MAQC) study

Emi Arikawa1, Yanyang Sun1, Jie Wang1, Qiong Zhou1, Baitang Ning2, Stacey L Dial2, Lei Guo2 and Jingping Yang1*

Author Affiliations

1 SuperArray Bioscience Corporation, Frederick, MD 21704, USA

2 National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA

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BMC Genomics 2008, 9:328  doi:10.1186/1471-2164-9-328

Published: 11 July 2008

Additional files

Additional file 1:

Comparison of performance metrics among the four quantitative platforms. The table shows the performance metrics of SYBR Green RT2 Profiler PCR Array, TaqMan PCR, StaRT-PCR and QuantiGene.

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Additional file 2:

Correlations between real-time PCR instruments for the raw CT values and fold-change results between the two MAQC reference RNA samples analyzed on the Human Drug Metabolism RT2Profiler PCR Array (APH-002). The scatter plots show the correlation comparison among three different models of real-time PCR instruments for the raw CT and fold-change results generated from the two MAQC reference RNA samples on the Human Drug Metabolism RT2Profiler PCR Arrays.

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Additional file 3:

The concordance of fold changes between SYBR Green-based RT2Profiler PCR Array and microarray platforms. The individual scatter plots for the comparison between the RT2Profiler PCR Array and each of the five microarrays are provided for the data presented in Figure 4 and Table 4 of the manuscript.

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