Confirmation of relative expression levels of the transcripts selected from the microarray analysis with semi quantitative RT-PCR (sqRT-PCR) or Northern blot hybridizations. Expression changes of 5 selected genes were determined by sqRT-PCR, microarray and Northern blot. The signal intensity of each transcript was normalized using At3g18780 (Actin 2). The Arabidopsis Genome Initiative (AGI) locus identifier of each gene is provided. The y-axis indicates the normalized expression level of the transcript. The x-axis represents hours post transfection or days post inoculation. Expression ratios are the average of three independent hybridizations ± standard deviation (SD). hpt, hours post transfection; dpi, days post inoculation. The error bars represent the standard deviation for the signals from each of the three independent hybridizations.
Babu et al. BMC Genomics 2008 9:325 doi:10.1186/1471-2164-9-325