Systemic infection and detection of PPV in Arabidopsis thaliana plants. (A) Symptoms on mock-inoculated (left) and PPV-infected (right) plants 17 days post inoculation (dpi). (B) RT-PCR and ELISA analysis of PPV-infected and mock-inoculated leaf tissues at 17 dpi. BR1, BR2, BR3, represents three independent biological replicates of PPV-infected and mock-inoculated leaf tissues, respectively. RT-PCR amplification of a 467 bp fragment corresponding to a segment of the PPV genome (nt 9140 to 9607), as tested using 25 cycles of amplification. +, ELISA positive; -; ELISA negative. (C) sqRT-PCR amplification of a cDNA fragment of the PPV genome isolated from Arabidopsis protoplasts transfected with pPPV-SK68 and pPPV-SK68Δ at 3, 6 and 12 hours post transfection (hpt), respectively. Histone 3 gene was used as a loading control. RT-PCR amplification for both panel B and C were carried out using the same RNA samples that were used in microarray hybridizations.
Babu et al. BMC Genomics 2008 9:325 doi:10.1186/1471-2164-9-325