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Open Access Highly Accessed Research article

Differential gene expression of the honey bee Apis mellifera associated with Varroa destructor infection

M Navajas1*, A Migeon1, C Alaux2, ML Martin-Magniette34, GE Robinson2, JD Evans5, S Cros-Arteil1, D Crauser6 and Y Le Conte6

Author Affiliations

1 INRA, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus International de Baillarguet, CS 30016, F-34988 Montferrier-sur-Lez Cedex, France

2 Institute for Genomic Biology, Department of Entomology, University of Illinois at Urbana-Champaign, 505 S. Goodwin Ave., Urbana, IL 61801, USA

3 INRA, UMR AgroParisTech/INRA MIA 518, 16 rue Claude Bernard, F-75231 Paris Cedex05, France

4 INRA, UMR INRA/1165/CNRS 8114/UEVE Unité de Recherche en Génomique Végétale, 2 rue Gaston Crémieux, F-91057 Evry Cedex, France

5 USDA-ARS Bee Research Laboratory, BARC-E Bldg 476, Beltsville, MD 20705, USA

6 INRA, UMR 406 Ecologie des Invertébrés, Site Agroparc, 84914 Avignon Cedex 9, France

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BMC Genomics 2008, 9:301  doi:10.1186/1471-2164-9-301

Published: 25 June 2008

Abstract

Background

The parasitic mite, Varroa destructor, is the most serious pest of the western honey bee, Apis mellifera, and has caused the death of millions of colonies worldwide. This mite reproduces in brood cells and parasitizes immature and adult bees. We investigated whether Varroa infestation induces changes in Apis mellifera gene expression, and whether there are genotypic differences that affect gene expression relevant to the bee's tolerance, as first steps toward unravelling mechanisms of host response and differences in susceptibility to Varroa parasitism.

Results

We explored the transcriptional response to mite parasitism in two genetic stocks of A. mellifera which differ in susceptibility to Varroa, comparing parasitized and non-parasitized full-sister pupae from both stocks. Bee expression profiles were analyzed using microarrays derived from honey bee ESTs whose annotation has recently been enhanced by results from the honey bee genome sequence. We measured differences in gene expression in two colonies of Varroa-susceptible and two colonies of Varroa-tolerant bees. We identified a set of 148 genes with significantly different patterns of expression: 32 varied with the presence of Varroa, 116 varied with bee genotype, and 2 with both. Varroa parasitism caused changes in the expression of genes related to embryonic development, cell metabolism and immunity. Bees tolerant to Varroa were mainly characterized by differences in the expression of genes regulating neuronal development, neuronal sensitivity and olfaction. Differences in olfaction and sensitivity to stimuli are two parameters that could, at least in part, account for bee tolerance to Varroa; differences in olfaction may be related to increased grooming and hygienic behavior, important behaviors known to be involved in Varroa tolerance.

Conclusion

These results suggest that differences in behavior, rather than in the immune system, underlie Varroa tolerance in honey bees, and give an indication of the specific physiological changes found in parasitized bees. They provide a first step toward better understanding molecular pathways involved in this important host-parasite relationship.