Additional file 3.
Suppression of tet-inducible transgene Pou5f1 in ZHBTc4 ES cells in a time course after adding tetracycline to the media. (A) Log expression change from microarray data (log10), oligo is in ORF. (B) Expression change in real-time PCR data normalized by expression in parental EB5 cell line, primers are in transgene-specific region. (C) Western blot showing decrease in POU5F1 protein amount, UBTF is used as a control.
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Sharov et al. BMC Genomics 2008 9:269 doi:10.1186/1471-2164-9-269