Table 2 |
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|
Fluorescence induction of P. luminescens carrying different promoter-reporter gene fusions in presence of insect larvae homogenate |
||
| Promoter of gene/operon |
Putative function of the gene product(s) |
Fluorescence Induction |
|
|
||
| Toxins |
||
| tccC1 (plu4167) |
insecticidal toxin complex TccC1 |
10.2-fold (± 2.0) |
| plu1645 |
similar to photopexin A und B (hemopexin-domain) |
10.1-fold (± 4.5) |
| plu2400 |
homologous to C-terminal region of dermonecrotic toxin (DNT) of Pasteurella multocida |
5.5-fold (± 1.9) |
| plu4122 |
contains Fascin domain, function unknown |
6.3-fold (± 2.8) |
| Metabolism |
||
| agaZSVCD/plu0839/gatY |
3,3-fold (± 0.3) |
|
| agaZ (plu0833) |
tagatose-6-phosphate-Kinase |
|
| agaS (plu0834) |
tagatose-6-phosphate ketose/aldose isomerase |
|
| agaV (plu0835) |
PTS-system, N-acetylgalactosamine-specific IIB component 2 (EIIB-AGA') |
|
| agaC (plu0836) |
PTS-system, N-acetylgalactosamine-specific IIC component 1 (EIIC-AGA) |
|
| agaD (plu0837) |
PTS-System, N-acetylgalactosamine-specific IID component (EIID-AGA) |
|
| plu0838 |
unknown, putative PTS permease |
|
| gatY (plu0839) |
tagatose-bisphosphate aldolase GatY |
|
| eutABC |
3.5-fold (± 1.0) |
|
| eutA (plu2969) |
ethanolamine degradation |
|
| eutB (plu2970) |
ethanolamine-ammonia-lyase heavy chain |
|
| eutC (plu2971) |
ethanolamine-ammonia-lyase light chain |
|
| hutUH |
2.5-fold (± 1.1) |
|
| hutU (plu3193) |
urocanate hydratase (urocanase) |
|
| hutH (plu3192) |
histidine ammonia lyase (histidase) |
|
| sucABCD |
1.5-fold (± 0.4) |
|
| sucA (plu1430) |
α-oxoglutarate dehydrogenase E1 component |
|
| sucB (plu1431) |
dihydrolipoamid succinyltransferase component of α-oxoglutarate dehydrogenase complex
(E2) |
|
| sucC (plu1432) |
succinyl-CoA synthetase β chain |
|
| sucD (plu1433) |
succinyl-CoA synthetase α-chain |
|
| plu1864 |
similarities to phosphoenolpyruvat phosphomutase |
1.0-fold (± 0.3) |
| plu2604 |
similar to glutarredoxin Protein YdhD |
5.0-fold (± 1.9) |
| Regulation |
||
| mlc (plu2226) |
„making large colonies" protein, involved in regulation of sugar metabolism |
4.8-fold (± 1.7) |
| DNA-modification |
||
| isplu16A (plu3160) |
transposase, IS630 family |
5.1-fold (± 1.0) |
| plu1165 |
putative relaxase |
5.5-fold (± 2.0) |
| plu3688 |
putative integrase/recombinase |
7.5-fold (± 3.1) |
| Synthesis of cell structure |
||
| spr (plu2864) |
lipoprotein spr precursor |
3.0-fold (± 1.0) |
| plu2790 |
similar to N-acetylmuramoyl-L-alanine amidase YbjR precursor of Escherichia coli |
4.7-fold (± 1.3) |
| plu1463 |
similar to DNA inversion product and tail-fiber protein of lambdoid prophage |
6.5-fold (± 1.9) |
| Transport, antibiotic resistance |
||
| plu4229 |
multidrug-resistance permease |
2.4-fold (± 0.7) |
| plu1579 |
similar to bacitracin permease of E. coli |
4.4-fold (± 1.4) |
| Antibiotic synthesis |
||
| plu3567-3561 |
3.8-fold (± 1.2) |
|
| plu3567 |
similar to N-formimidoyl fortimicin A synthase |
|
| plu3566 |
similar to putative methylase and protoporphyrinogen oxidase |
|
| plu3565 |
similar to class II aminotransferase and 5-aminolevulinic acid synthase |
|
| plu3564 |
weakly similar to PapB protein and to chorismate mutase/prephenate dehydrogenase |
|
| plu3563 |
similar to p-aminobenzoic acid synthase |
|
| plu3562 |
similar to dehydrogenase PapC of Streptomyces pristinaespiralis |
|
| plu3561 |
probable transport protein |
|
| Unknown function |
||
| plu0801 |
PrK012399-domain, similar to Plu1012 and Plu1017 |
2.0-fold (± 0.3) |
| plu1012-1010 |
10.6-fold (± 4.2) |
|
| plu1012 |
PrK012399-domain, similar to Plu0801 and Plu1017 |
|
| plu1011 |
„TIM-br_sig_trns"-Domain, putative sigma54-dependent transcriptional activator |
|
| plu1010 |
unknown |
|
| plu1017 |
PrK012399-domain, similar to Plu0801 and Plu1012 |
6.6-fold (± 4.6) |
| plu1672 |
similar to Plu1691 of Photorhabdus, function unknown |
2.0-fold (± 0.8) |
| plu1950 |
similar to Plu2538 of Photorhabdus, function unknown |
3.0-fold (± 0.9) |
| plu2652 |
function unknown |
4.0-fold (± 1.5) |
| plu2809 |
similar to protein YcfD of E. coli, contains JmjC domain (pot. metalloenzym) |
3.0-fold (± 1.1) |
| plu3479 |
unknown |
1.1-fold (± 0.2) |
| plu3608 |
similar to protein Plu3387 of Photorhabdus, function unknown |
4.5 (± 0.3) |
| Controls |
||
| mcf (plu4142) |
"makes caterpillars floppy" toxin |
6.0 (± 2.3) |
| mcf2 (plu3128) |
"makes caterpillars floppy" toxin 2 |
0.9 (± 0.2) |
| tcdA1 (plu0962) |
insecticidal toxin complex TcdA1 |
6.5 (± 1.0) |
| tcaA/B (plu0516) |
insecticidal toxin complex TcaA/TcaB |
1.25 (± 0.2) |
| tcaZ (plu0514) |
insecticidal toxin complex TcaZ |
1.3 (± 0.3) |
| tccA1 (plu4169) |
insecticidal toxin complex TccA1 |
1.6 (± 0.2) |
| prtA (plu0655) |
alkaline metalloprotease PrtA |
2.1-fold (± 0.7) |
|
|
||
|
P. luminescens carrying the indicated reporter gene plasmid was grown in complex medium and in complex medium containing insect homogenate (in vitro). Each single reporter strain was tested on differential fluorescence induction in presence of insect homogenate. In the left panel, the genes or operons located downstream of the promoters tested are listed, along with putative functions for the gene products (middle panel). At the right panel, the fluorescence induction factors in presence of G. mellonella insect homogenate are shown. Enhanced activity is emphasized by bold numbers. Experimental standard deviation of at least three independent measurements is presented (in brackets). |
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|
Münch et al. BMC Genomics 2008 9:229 doi:10.1186/1471-2164-9-229 |
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